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University of Illinois at Urbana-Champaign
Structural Characterization of Liquid-Chromatographic Effluents via Reverse File-Searching of Fluorescence Spectra
Abstract
dc:descriptionThe work described here has shown the feasibility of combining the technique of liquid chromatography, fluorescence detection and computerized file-searching to produce a new approach to mixture analysis. The technique operates by separating mixture components in a liquid-chromatograph and submitting them sequentially to a high-performance spectrofluorometer for spectral characterization. The excitation and emission fluorescence spectra are searched against a library containing more than 1150 compounds, by a new algorithm which compares spectral similarity from the point of view of both the unknown spectrum and the library spectrum, thus combining the best features of forward and reverse searching.
Degree
thesis:*- Name thesis:degree_name
- Ph.D.
- Level thesis:degree_level
- Dissertation
- Discipline thesis:degree_discipline
- Chemistry
- Grantor
- University of Illinois at Urbana-Champaign
- Year dc:date
- 2014
Author and committee
dc:creator, dc:contributor.*- Author dc:creator
-
- Hardesty, Patrick Thomas
Subjects
dc:subject × 1Rights
- Language dc:language
- eng
Identifiers
dc:identifier.*- Identifier
- (UMI)AAI8108532
- OAI identifier oai:identifier
- oai:www.ideals.illinois.edu:2142/67241