Mid infrared spectroscopic imaging and tomography

Fourier Transform Infrared (FT-IR) Spectroscopic Imaging is an optical technique that simultaneously provides morphological (two dimensional) and mid-infrared spectral (one dimensional) information for a specimen under observation. In this dissertation, we develop a new instrument that extends the capabilities of FT-IR imaging to provide three dimensional morphological information along with mid-infrared spectral information on the microscopic length scale. We first demonstrate that spectroscopic information from the mid-infrared can be used to perform histologic recognition and cancer detection in prostate, breast and skin cancer tissues. These studies utilize FT-IR imaging data of thin tissue slices from several hundred patients. The segmentation algorithms and instrumentation used are described in detail. We perform a rigorous analysis of instrumentation and data so as to understand the limits and limitations of current state-of-the-art instrumentation. We then propose a new instrument that adds a new dimension of information to current instruments and establish the new state-of-the-art in mid-infrared imaging. The theoretical basis of such instrumentation is presented in detail and techniques for visualization of four dimensional data are outlined.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Bioengineering

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2013

Author dc:creator

• Reddy, Rohith

Contributors dc:contributor

• Bhargava, Rohit
• Carney, Paul S.
• Do, Minh N.
• Ahuja, Narendra

• Fourier Transform Infrared (FT-IR) spectroscopic Imaging
• Mid-Infrared Spectroscopic Tomography
• Spectroscopic Imaging
• Spectroscopic Tomography
• mid-Infrared (Mid-IR)
• Spectroscopy
• Imaging

Statement dc:rights

• Copyright 2013 Rohith Reddy

Language dc:language

en

Handle dc:identifier

http://hdl.handle.net/2142/44759

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/44759