High resolution optical DNA mapping

Many types of diseases including cancer and autism are associated with copy-number variations in the genome. Most of these variations could not be identified with existing sequencing and optical DNA mapping methods. We have developed Multi-color Super-resolution technique, with potential for high throughput and low cost, which can allow us to recognize more of these variations. Our technique has made 10–fold improvement in the resolution of optical DNA mapping. Using a 180 kb BAC clone as a model system, we resolved dense patterns from 108 fluorescent labels of two different colors representing two different sequence-motifs. Overall, a detailed DNA map with 100 bp resolution was achieved, which has the potential to reveal detailed information about genetic variance and to facilitate medical diagnosis of genetic disease.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Biophysics & Computnl Biology

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2013

Author dc:creator

• Baday, Murat

Contributors dc:contributor

• Selvin, Paul R.
• Schroeder, Charles M.
• Nayfeh, Munir H.
• Myong, Su-A
• Kuhlman, Thomas E.

• copy-number variations
• Single molecule-High Resolution Imaging with Photobleaching (SHRImP)
• High-Resolution Co-localization (SHREC)
• Deoxyribonucleic Acid (DNA)
• Super-resolution
• optical DNA mapping
• genomic variations

Statement dc:rights

• Copyright 2013 Murat Baday

Language dc:language

en

Handle dc:identifier

http://hdl.handle.net/2142/44261

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/44261