In vitro separation of chimeral pears into their component genotypes

An adventitious shoot regeneration system from leaf explants of several pear cultivars has been developed. Leaves were excised from in vitro grown shoots, transversely cut into halves, and plated onto Petri dishes containing regeneration media. Cultures were kept in the dark for three weeks before adventitious shoots were observed. The effect of different levels of Thiadizuron (TDZ) and Naphthaleneacetic acid (NAA) on shoot regeneration was investigated. Among the different levels of TDZ used, a range of 3 to 7 uM was optimum for most cultivars, while a level of 4.5 to 5 uM NAA gave the best regeneration for the two cultivars, 'Seckel' and 'Louise Bonne Panachee'. The effect of different salts on shoot regeneration was also investigated. Nitsch and Nitsch (1969) salts gave the highest regeneration percentage. It was also found that both nitrogen level and form in the medium played an important role in adventitious shoot formation.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Agriculture, Plant Culture

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2011

Author dc:creator

• Abu-Qaoud, Hassan Ahmed

Contributors dc:contributor

• Skirvin, Robert M.

• Agriculture, Plant Culture

Statement dc:rights

• Copyright 1989 Abu-Qaoud, Hassan Ahmed

Language dc:language

eng

Identifier

(UMI)AAI9010788
AAI9010788

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/23686