University of Illinois at Urbana-Champaign
Studies on CheA(B): Phosphorylation, methylation and behavioral control during chemotactic signal transduction in Bacillus subtilis
Abstract
dc:descriptionChemotaxis in Bacillus subtilis is a prototypical behavior and signal transduction process, and CheA$\sb{\rm B}$ plays an important role in it. The $cheA\sb{\rm B}$ gene was isolated, sequenced, expressed and mutated. It encoded a large negatively charged protein with a molecular weight of approximately 74,000. The predicted protein sequence has 33% homology with the Escherichia coli and CheA$\sb{\rm E}$. Such proteins have been found to autophosphorylate and are members of the same histidine kinase signal modulating family. CheA$\sb{\rm B}$ has several conserved regions (including the histidine that is phosphorylated in CheA$\sb{\rm E}$) that coincide with the other autophosphorylated signal transducers. In vitro experiments on partially purified CheA$\sb{\rm B}$ indicates that it also appears to autophosphorylate. CheA$\sb{\rm E}$ mediates the effect of binding of attractants or repellents at the MCPs (receptors) by altering its rate of autophosphorylation and subsequent phosphorylation of CheY, to bring about behavioral change, and CheB, to bring about adaptation. CheA$\sb{\rm B}$ appears to play a similar central controlling role, for a null mutant and all point mutants tested were defective in attractant-induced methanol production and showed no behavioral response upon addition of chemoeffectors. Furthermore, methylation of the MCPs, in which methyl groups are transferred away to an acceptor and replaced by methyl groups from S-adenosylmethionine, is regulated by CheB$\sb{\rm B}$, whose activity is controlled by CheA$\sb{\rm B}$.
Degree
thesis:*- Name thesis:degree_name
- Ph.D.
- Level thesis:degree_level
- Dissertation
- Discipline thesis:degree_discipline
- Biology, Molecular
- Grantor
- University of Illinois at Urbana-Champaign
- Year dc:date
- 2011
Author and committee
dc:creator, dc:contributor.*- Author dc:creator
-
- Fuhrer, Douglas Keith
- Contributors dc:contributor
-
- Ordal, George W.
Subjects
dc:subject × 3Rights
dc:rights- Statement dc:rights
-
- Copyright 1992 Fuhrer, Douglas Keith
- Language dc:language
- eng
Identifiers
dc:identifier.*- Identifier
-
AAI9305529
(UMI)AAI9305529 - OAI identifier oai:identifier
- oai:www.ideals.illinois.edu:2142/23616