University of Illinois at Urbana-Champaign
Molecular analyses of differences in gene expression due to changes in the level of dietary fat
Abstract
dc:descriptionDietary fat has been shown to exacerbate important chronic diseases including, heart disease, obesity, hyperlipidemia, diabetes and cancer. This thesis described studies carried out to further our understanding of genes whose expression is altered by the level of dietary fat. The experimental approach was designed to obtain basic information in virgin female mice whose responses to dietary fat were not confounded by the production of a mammary tumor virus, hormonal events of pregnancy or the use of a chemical carcinogen. I have used molecular biology techniques to identify genes regulated by different levels of dietary fat. cDNA libraries were constructed from mammary tissue of virgin female Balb/c mice (7wks) fed a fat free diet for one week and then switched to either a 3 or 20% corn oil (co) diet for 2 wks. Subtractive hybridization of the lowfat cDNA library revealed genes expressed preferentially by mice fed the 3% co diet. These clones are designated lowfat mammary 1 and 2 (LFM-1, LFM-2). The diet regulated LFM-1 is a homolog to an UV-inducible gene. Since DNA repair enzymes are also induced by UV-irradiation, the identification of this single gene suggests that genes involved in DNA repair also may be regulated by dietary fat. Regulation of DNA repair genes by different levels of dietary fat may provide the first experimental link between the high fat diet and promotion of specific diseases.
Degree
thesis:*- Name thesis:degree_name
- Ph.D.
- Level thesis:degree_level
- Dissertation
- Discipline thesis:degree_discipline
- Biology, Molecular
- Grantor
- University of Illinois at Urbana-Champaign
- Year dc:date
- 2011
Author and committee
dc:creator, dc:contributor.*- Author dc:creator
-
- Elliott, Timothy Scott
- Contributors dc:contributor
-
- Visek, Willard J.
Subjects
dc:subject × 2Rights
dc:rights- Statement dc:rights
-
- Copyright 1991 Elliott, Timothy Scott
- Language dc:language
- eng
Identifiers
dc:identifier.*- Identifier
-
AAI9124408
(UMI)AAI9124408 - OAI identifier oai:identifier
- oai:www.ideals.illinois.edu:2142/23591