Sequence-specific recognition of DNA by RSRI endonuclease of Rhodobacter sphaeroides, an isoschizomer of EcoRI

In order to study the interaction of RsrI endonuclease with its DNA recognition sequence and to determine whether RsrI recognizes DNA using a mechanism similar to that of EcoRI, I have purified milligram amounts of the enzyme to near homogeneity from its natural source, Rhodobacter sphaeroides strain 630. The enzyme comigrates with EcoRI during SDS-PAGE, with a reduced and denatured molecular weight of 30,000 $\pm$ 2000 Da. RsrI endonuclease is a dimer at concentrations of 0.05 to 1.4 mg/ml. In contrast to EcoRI, no tetrameric form was observed. The isoelectric point of the endonuclease was determined to be 7.0-different than the value of 6.3 reported for EcoRI.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Chemistry, Biochemistry

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2011

Author dc:creator

• Aiken, Christopher Robin

Contributors dc:contributor

• Gumport, Richard I.

• Chemistry, Biochemistry

Statement dc:rights

• Copyright 1991 Aiken, Christopher Robin

Language dc:language

eng

Identifier

AAI9124375
(UMI)AAI9124375

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/23553