Enzymatic hydrolysis of liquefied corn starch in a membrane bioreactor

The overall objective of this project was to develop and optimize a membrane reactor for the continuous saccharification of liquefied corn starch by glucoamylase. The effects of enzyme concentration, substrate concentration and residence time on starch conversion were studied. Increasing the concentration of enzyme (AMG 200L, NOVO Laboratories) increased conversion at all substrate levels studied (1-30% w/v) up to an enzyme level of 6 g/L, but appeared to be unaffected at higher enzyme concentrations. Substrate conversions of 95% or greater were consistently obtained at substrate-to-enzyme ratios (S:E) of 25-50 w/w. Lower S:E ratios enhanced the reversion reaction and lowered the glucose content of the product stream. At equivalent substrate and enzyme concentrations, conversions were 15-22% greater as the residence time was increased from 2 to 3 hours.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Food Science

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2011

Author dc:creator

• Sims, Kevin Allen

Contributors dc:contributor

• Cheryan, Munir

• Agriculture, Food Science and Technology
• Engineering, Agricultural

Statement dc:rights

• Copyright 1990 Sims, Kevin Allen

Language dc:language

eng

Identifier

AAI9021757
(UMI)AAI9021757

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/23514