Transcriptional regulation of the Bacillus subtilis pyrimidine biosynthetic operon

A complete transcript of the Bacillus subtilis pyr operon contains the following elements in 5$\sp\prime$ to 3$\sp\prime$ order: a 150 nt untranslated leader; pyrR, which encodes a 21 kDa protein; a 173 nt intercistronic region; pyrP, which encodes a 46 kDa protein; a 145 nt intercistronic region; followed by 8 overlapping cistrons encoding all of the 6 enzymes for de novo pyrimidine biosynthesis. Transcription is controlled by the availability of pyrimidines via a PryR-mediated attenuation mechanism at 3 attenuators within the operon, which are located in the 5$\sp\prime$ leader, the pyrP-pyrP intercistronic, and the pyrP-pyrB intercistronic regions. Each of the attenuators is predicted to be comprised of an antiterminator (AT) stem-loop structure, a terminator (T) stem-loop structure which overlaps with the 3$\sp\prime$ strand of the AT, and a consensus sequence which is proposed to be the binding site for the regulatory protein PyrR, and is also capable of forming an anti-antiterminator (AAT) stem-loop structure that competes with the 5$\sp\prime$ strand of the AT.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Biochemistry

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2011

Author dc:creator

• Lu, Yang

Contributors dc:contributor

• Switzer, Robert L.

• Biology, Molecular
• Biology, Microbiology
• Chemistry, Biochemistry

Statement dc:rights

• Copyright 1996 Lu, Yang

Language dc:language

eng

Identifier

9780591088618
AAI9702590
(UMI)AAI9702590

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/23445