Soybean transformation and analysis of transformed tissues using PCR

Factors which affect the transformation efficiency of Agrobacterium tumefaciens-mediated soybean transformation were studied. It was found that pre-induction of A. tumefaciens A281 using acetosyringone significantly increased the efficiency of transformation and this could be boosted further by octopine. The application of these pre-induction conditions led to the improvement in recovery of putative transformed soybean shoots although no fertile plants were recovered.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Agronomy

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2011

Author dc:creator

• Luo, Guangbin

Contributors dc:contributor

• Widholm, Jack M.

• Biology, Genetics

Statement dc:rights

• Copyright 1993 Luo, Guangbin

Language dc:language

eng

Identifier

AAI9314908
(UMI)AAI9314908

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/23344