Expression of single chain antibody-encoding genes in fission yeast and tobacco

The goal of this project was to explore the expression of genes encoding single chain antibodies (SCA) in eukaryotic cells. During the course of this research, a new PCR-based strategy was developed that significantly reduced the time required for SCA gene synthesis. Although initially developed for the synthesis of SCA genes from templates of known sequence, PCR primers have now been developed that have universal applicability for this strategy. It was established that murine codon bias did not preclude efficient expression of SCA genes in E. coli.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Plant Biology

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

1992

Author dc:creator

• Davis, George Thomas

Contributors dc:contributor

• Jacobs, Thomas W.

• Biology, Molecular
• Biology, Genetics

Statement dc:rights

• Copyright 1992 Davis, George Thomas

Language dc:language

eng

Identifier

AAI9305501
(UMI)AAI9305501

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/23325