Biochemical and molecular characterization of chemotaxis genes in Bacillus subtilis

The cheF gene, an essential gene for chemotaxis in B. subtilis was cloned, localized and characterized. This gene is contained in a 7.7 kb EcoR1 DNA fragment that was isolated from a $\lambda$ Charon 4A B. subtilis chromosomal DNA library. A 0.7 kb Pst 1 DNA fragment of this 7.7 kb EcoR1 fragment was subcloned into an expression vector, pSI-1 and shown to complement the cheF mutation both for chemotaxis and for methanol formation in response to the addition of attractants.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Biochemistry

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2011

Author dc:creator

• Ying, Ching-Wen

Contributors dc:contributor

• Ordal, George W.

• Chemistry, Biochemistry

Statement dc:rights

• Copyright 1990 Ying, Ching-Wen

Language dc:language

eng

Identifier

AAI9026361
(UMI)AAI9026361

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/22824