Experimental and computational studies of receptor-mediated cell detachment in shear flow

Specific, receptor-mediated adhesion of cells to ligand-coated surfaces in viscous shear flow is a central phenomenon in many physiological and biotechnological processes. Mechanical strength of specific adhesion is generally presumed to be related to chemical affinity of receptor/ligand bonds, but no experimental study has been previously directed toward this issue. I investigate the dependence of receptor/ligand adhesion strength on bond affinity using a radial fluid flow chamber assay to measure the force needed to detach polystyrene beads covalently coated with immunoglobulin G (IgG) from glass surfaces covalently coated with protein A (SpA). A spectrum of animal species sources for IgG permits examination of three decades of SpA/IgG binding affinity. The results for the model cell system demonstrate that bond strength varies with the logarithm of the binding affinity, providing the first experimental support for theoretical treatments of this relationship.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Chemical and Biomolecular Engineering

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2011

Author dc:creator

• Kuo, Suzanne Chang

Contributors dc:contributor

• Lauffenburger, Douglas A.

• Biology, Cell
• Engineering, Biomedical
• Engineering, Chemical

Statement dc:rights

• Copyright 1994 Kuo, Suzanne Chang

Language dc:language

eng

Identifier

AAI9512441
(UMI)AAI9512441

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/22745