Antimicrobial pharmacodynamics: The gentamicin - Escherichia coli relationship

The purpose of this thesis was to: (1) explore the in vitro dose-response relationship between gentamicin and Escherichia coli under conditions of constant and declining antibiotic concentrations and (2) to characterize the kinetics of the relationship in an attempt to better predict antimicrobial efficacy. A unique in vitro dynamic culture system was constructed to subject bacteria growing in broth culture to drug concentrations simulating in vivo gentamicin exposure. Changes in viability were monitored as a function of antibiotic concentration and duration of exposure following continuous and IV bolus dose simulations. Significant bacterial killing first occurred at gentamicin concentrations equal to 1/2 the MIC. Exposure to drug concentrations between 1/2 and 2 $\times$ the MIC resulted in a 99.9% loss of viability, followed by resistant regrowth within 4 hours. Resistant bacteria were also identified after single IV bolus dose exposure. Drug resistance was increased up to 8 $\times$ the original MIC and was unstable in antibiotic-free media.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Comparative Biosciences

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2011

Author dc:creator

• Kilroy, Carolyn S.

Contributors dc:contributor

• Bevill, R.F.

• Biology, Microbiology
• Health Sciences, Pharmacology

Statement dc:rights

• Copyright 1995 Kilroy, Carolyn S.

Language dc:language

eng

Identifier

AAI9624387
(UMI)AAI9624387

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/22515