Evaluation of the induction of rat hepatic and pancreatic glutathione S-transferases by treatment with the cruciferous nitrile, cyanohydroxybutene

Cyanohydroxybutene (CHB) is a nitrile derived from glucosinolates commonly found in cruciferous plants. Glucosinolate derivatives are believed to provide protection against carcinogens by inducing detoxifying including the glutathione S-transferase isoenzymes (GSTs). The GSTs catalyze the conjugation of glutathione (GSH) to electrophilic xenobiotics. As peroxidases, they detoxify organic hydroperoxides. Each isoenzyme has a unique level of activity in detoxifying xenobiotics, including certain carcinogens. The amount of individual isoenzymes within tissues and cells, particularly in response to treatment with inducers, is important when considering mechanisms of cancer chemoprevention and chemotherapy. At 200 mg/kg per os, CHB is selectively toxic to the rat exocrine pancreas. At this and non-toxic doses, CHB causes the sustained elevation of GSH in the liver and pancreas. The elevation, which follows a brief depletion phase, is associated with the induction of the hepatic GSH synthesizing enzyme, γ-glutamylcysteine synthetase, and an increase in the cellular uptake of GSH precursors. CHB also induces hepatic and pancreatic GST and quinone reductase activities and hepatic GSH reductase activity.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Comparative Biosciences

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2011

Author dc:creator

• March, Thomas Hugh

Contributors dc:contributor

• Wallig, Matthew A.

• Health Sciences, Toxicology
• Health Sciences, Pathology

Statement dc:rights

• Copyright 1996 March, Thomas Hugh

Language dc:language

eng

Identifier

9780591199338
AAI9712368
(UMI)AAI9712368

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/22232