University of Illinois at Urbana-Champaign
Isolation and characterization of cytochrome P450s from Papilio polyxenes and Papilio glaucus
Abstract
dc:descriptionPrevious studies have isolated two cytochrome P450 monooxygenase cDNAs designated CYP6B1v1 and CYP6B1v2 from the oligophagous Papilio polyxenes. Baculovirus-mediated expression of these P450s demonstrated that both enzymes metabolize substantial amounts of linear furanocoumarins and low, if any, amount of angular furanocoumarins. The polyphagous Papilio glaucus rarely encounters furanocoumarin-containing plants and yet it contains low constitutive levels of cytochrome P450s capable of metabolizing furanocoumarins, which significantly induce these cytochrome P450 activities when present furanocoumarins in the diet. The purposes of this thesis were to isolate and characterize cDNAs and genes encoding an angular furanocoumarin-metabolizing P450 from P. polyxenes and furanocoumarin-metabolizing P450s from P. glaucus.
Degree
thesis:*- Name thesis:degree_name
- Ph.D.
- Level thesis:degree_level
- Dissertation
- Discipline thesis:degree_discipline
- Entomology
- Grantor
- University of Illinois at Urbana-Champaign
- Year dc:date
- 2011
Author and committee
dc:creator, dc:contributor.*- Author dc:creator
-
- Hung, Chien-Fu
- Contributors dc:contributor
-
- Berenbaum, May R.
Subjects
dc:subject × 2Rights
dc:rights- Statement dc:rights
-
- Copyright 1996 Hung, Chien-Fu
- Language dc:language
- eng
Identifiers
dc:identifier.*- Identifier
-
9780591199086
AAI9712318
(UMI)AAI9712318 - OAI identifier oai:identifier
- oai:www.ideals.illinois.edu:2142/22188