Macrophage differentiation: A heterogeneous theory

"Functional heterogeneity of macrophage populations has been characterized however, questions remain regarding the mechanisms underlying the development of this heterogeneity. A procedure was developed to molecularly phenotype colonies of bone marrow-derived macrophage(s) (BMDM). Gene expression from individual clones was monitored using the reverse transcription polymerase chain reaction (RT-PCR) to permit simultaneous amplification of multiple specific gene transcripts. Internal ""nested"" primers were utilized in addition to the two traditional external primers thus, increasing the reliability of amplification. Results revealed hierarchal expression of macrophage-associated genes. Predominant colony phenotypes observed were unique both for the period of differentiation and choice of hematopoietic stimulus. Furthermore, colonies were subcloned into different growth factors and phenotyped on sequential days demonstrating that individual clones were not committed to specific phenotypes but retained the capacity to express different functional phenotypes."

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Animal Sciences

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2011

Author dc:creator

• Witsell, Alice Louise

Contributors dc:contributor

• Schook, Lawrence B.

• Biology, Cell
• Health Sciences, Immunology

Statement dc:rights

• Copyright 1992 Witsell, Alice Louise

Language dc:language

eng

Identifier

AAI9236624
(UMI)AAI9236624

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/22151