Enhanced chemiluminescent immunoassay for staphylococcal enterotoxin B in foods

An enhanced chemiluminescent immunoassay (CLIA) for the detection of staphylococcal enterotoxin B (SEB) in foods was developed. This assay utilizes the $\rho$-iodophenol enhanced oxidation of luminol by hydrogen peroxide as indicator reaction. The reaction was catalyzed by horseradish peroxidase (HRPO) conjugated to immunospecific anti-SEB immunoglobulin G (IgG). Addition of $\rho$-iodophenol to the reaction mixture resulted in $>$2,000 fold increase in light emission over the unenhanced reaction. Furthermore, light emission was not only of high intensity but also of prolonged duration. This simplified manipulation since it was not necessary to start the reaction in front of the photodetector.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Food Science

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2011

Author dc:creator

• Ocasio, Wilfredo

Contributors dc:contributor

• Blaschek, Hans-Peter M.

• Agriculture, Food Science and Technology
• Biology, Microbiology
• Chemistry, Analytical
• Health Sciences, Public Health

Statement dc:rights

• Copyright 1989 Ocasio, Wilfredo

Language dc:language

eng

Identifier

AAI9010971
(UMI)AAI9010971

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/21961