Molecular, genetic, and biochemical studies of mannityl opine utilization by Agrobacterium tumefaciens: Its evolutionary implications

Agrobacterium strain NT1 harboring pSaB4, which contains the 14-kb BamHI fragment 4 from the octopine/mannityl opine-type Ti plasmid pTi15955, grows well with agropine (AGR). However, this strain grows slowly with mannopine (MOP) as sole carbon source, but gives rise to spontaneous mutants which grow as fast on this opine as does wild type cells. DNA sequence analysis of the region necessary for utilization of these opines revealed seven putative genes; moc (manniyl opine catabolism) D and E, oriented from right to left, and $mocRCBAR\sp\prime$, from left to right. MocD is a homologue of the anabolic conjugase encoded by mas2$\sp\prime$ required for MOP biosynthesis encoded on the T$\sb{\rm R}$ region of pTi15955. MocE and MocC have homologies with the amino half and the carboxyl half, respectively, of Mas1 (MOP reductase), the second enzyme for MOP biosynthesis encoded by mas1$\sp\prime$. MocR and MocR$\sp\prime$ are related to each other, and to the putative repressor for the AGR degradation system encoded by the agropine-type rhizogenic plasmid pRiA4. MocB and MocA are homologues of 6-phosphogluconate dehydratase and glucose-6-phosphate dehydrogenase, respectively.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Microbiology

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2011

Author dc:creator

• Kim, Kun-Soo

Contributors dc:contributor

• Farrand, Stephen K.

• Biology, Molecular
• Biology, Microbiology
• Chemistry, Biochemistry

Statement dc:rights

• Copyright 1995 Kim, Kun-Soo

Language dc:language

eng

Identifier

AAI9624391
(UMI)AAI9624391

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/21705