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University of Illinois at Urbana-Champaign
Development of in vitro regeneration and gene transfer systems for conifer species
Abstract
dc:descriptionIn vitro shoot establishment and proliferation of Sequoia sempervirens Endl. stem segments on a Wolter and Skoog (WS) medium supplemented with 15 μM zeatin was found to be optimum for shoot proliferation. Using in vitro-grown needles of S. sempervirens, shoot organogenesis and somatic embryogenesis were induced. The influence of genotype and various cytokinins (BA, BPA, and TDZ) in combination with 2,4-D were investigated. Among the cytokinins tested, 20 μM BPA with 5 μM 2,4-D produced the highest frequency of shoot organogenesis from three genotypes while, 10 μM TDZ with 5 μM 2,4-D produced the highest frequency of somatic embryos from two of three genotypes.
Degree
thesis:*- Name thesis:degree_name
- Ph.D.
- Level thesis:degree_level
- Dissertation
- Discipline thesis:degree_discipline
- Crop Sciences
- Grantor
- University of Illinois at Urbana-Champaign
- Year dc:date
- 2011
Author and committee
dc:creator, dc:contributor.*- Author dc:creator
-
- Sul, Ill-Whan
- Contributors dc:contributor
-
- Korban, Schuyler S.
Subjects
dc:subject × 3Rights
dc:rights- Statement dc:rights
-
- Copyright 1995 Sul, Ill-Whan
- Language dc:language
- eng
Identifiers
dc:identifier.*- Identifier
-
AAI9543739
(UMI)AAI9543739 - OAI identifier oai:identifier
- oai:www.ideals.illinois.edu:2142/21639