In vivo studies of metabolism and visual phototransduction of the isolated toad retina by phosphorus-31 nuclear magnetic resonance spectroscopy

Retinal physiology and metabolism, active areas of inquiry twenty years ago, have been relatively inactive recently. During the last twenty years, however, the development of nuclear magnetic resonance spectroscopy as a tool to study tissue metabolism, has changed the nature of the field of physiology. In this thesis I demonstrate the use of this technique to study several aspects of retinal metabolism and phototransduction. General considerations and the potential of the technique for studying the retina is presented in Chapter 3. With the improvements in the method of maintaining retinae (Chapter 4) I could conduct longer experiments than my preliminary studies. Thus, I measured the concentrations of phosphorus-metabolites to see if these change with light adaptation (Chapter 5) and if cGMP could be observed in vivo (Chapter 6).

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Biophysics

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2011

Author dc:creator

• Apte, Dipali V.

Contributors dc:contributor

• Ebrey, Thomas G.

• Biology, Neuroscience
• Biology, Animal Physiology
• Biophysics, General

Statement dc:rights

• Copyright 1992 Apte, Dipali V.

Language dc:language

eng

Identifier

AAI9236391
(UMI)AAI9236391

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/21623