Demonstration and quantitation of lactate in oxidative and glycolytic fibers of skeletal muscles

Two peaks consistently arise near 1.3 ppm in $\sp1$H NMR spectroscopic studies of fatiguing skeletal muscles. They are typically 15-20 Hz apart at 300 MHz. Known resonances in this region include lactate, alanine, threonine, and the acyl chain of lipid. From both NMR evidence (COSY, lactate editing, and $\sp{13}$C enrichment) and biochemical evidence (including results of electrical, caffeine or high potassium stimulation, and iodoacetate inhibition of glycolysis), both peaks are identified as lactate.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Biophysics

Grantor

University of Illinois at Urbana-Champaign

Date dc:date

10000-01-01

Author dc:creator

• Shen, Dee

Contributors dc:contributor

• Dawson, M. Joan

• Biology, Animal Physiology
• Biophysics, General

Statement dc:rights

• Copyright 1996 Shen, Dee

Language dc:language

eng

Identifier

AAI9625192
(UMI)AAI9625192

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/21475