Molecular cloning, immunolocalization, and transgenic expression in yeast and tobacco of three cDNAs that encode sugar transporters in sugar beet

Sugar transporter genes from sugar beet have been cloned as a first step in understanding their contribution to assimilate partitioning. Two cloning strategies were attempted. One was functional complementation of a yeast mutant with a sugar beet cDNA library constructed in a yeast expression vector. The other approach was PCR amplification based on sequence homologies found in a superfamily of sugar transporters. No promising clones were isolated by functional complementation. In contrast, several cDNA clones have been obtained by PCR amplification and subsequent screening of a cDNA library.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Biology

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2011

Author dc:creator

• Chiou, Tzyy-Jen

Contributors dc:contributor

• Bush, Daniel R.

• Biology, Molecular
• Biology, Botany
• Biology, Plant Physiology

Statement dc:rights

• Copyright 1995 Chiou, Tzyy-Jen

Language dc:language

eng

Identifier

AAI9522092
(UMI)AAI9522092

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/21331