Application of plant tissue and organ culture in soybean improvement for resistance to Septoria glycines

Culture filtrate of Septoria glycines Hemmi grown in Septoria medium contained a host-specific toxin which induced typical symptoms of brown spot disease on soybean leaves. Culture filtrate from 21-day-old cultures caused chlorosis on detached cotyledons and inhibited the growth of soybean callus. Less than 20% of the seeds germinated on culture filtrate-containing water-agar at a 1:2 dilution. Embryogenesis was stimulated when germinated soybean seeds on water-agar (6 g/l) containing 40 μM 2,4-dichlorophenoxy-acetic acid (2,4-D) were transferred to Murashige and Skoog (MS) medium containing 20 μM benzylaminopurine (BAP). Organogenesis was facilitated by transferring organogenic callus from MS medium with 20 μM BAP to MS medium with 1 μM BAP. Plants resistant to the pathotoxic culture filtrate of S. glycines were regenerated from immature embryo of soybean cultivar (cv.) BSR201 and from cotyledonary nodes of mature seeds of the cvs. BSR201, Fayette, and one breeding line, L1615. The incubation period for S. glycines in progeny of resistant plants (cv. BSR201) obtained from immature embryo was five weeks longer than the incubation period in susceptible plants. The resistant plants were small, but not all small plants were resistant. Variation was also observed in plant maturity, growth habit, and fertility.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Crop Sciences

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2011

Author dc:creator

• Song, Hee-Sook

Contributors dc:contributor

• Lim, Sung M.

• Agriculture, Plant Pathology

Statement dc:rights

• Copyright 1991 Song, Hee-Sook

Language dc:language

eng

Identifier

AAI9136741
(UMI)AAI9136741

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/21064