Analysis of ABA-regulated expression of the maize Glb1 gene in tobacco seeds and maize cells

The expression and regulation of the Glb1 gene was examined in both transgenic tobacco plants as well as maize P3377 cells. For transgenic studies, a ppGlb1GUS construct, consisting of 1402 bp 5$\sp\prime$ flanking sequence of Glb1, 1919 bp GUS (coding sequence, and 283 bp 3$\sp\prime$ Nos terminator, was cloned into Bin19 vector and introduced into tobacco plants by Agrobacterium-mediated transformation. Histochemical GUS assays of R$\sb{\rm o}$ tobacco mature seeds indicate that the Glb1 promoter drives GUS expression in ABA treated seeds, but not in water treated ones. Further GUS assays of the R$\sb1$ seeds at different developmental stages revealed that without ABA treatment, the Glb1 promoter drives GUS expression in immature seeds. The results from both R$\sb{\rm o}$ and R$\sb1$ tobacco plants indicated that GUS expression in tobacco is embryo specific. To study the accumulation of GLB1 proteins in tobacco plants, the plasmid ppConGlb1 was constructed by inserting the 2010 bp Glb1cDNA fragment into an expression cassette from the α\sp\prime-subunit of the soybean β-conglycinin gene consisting of 866 bp 5$\sp\prime$ flanking sequence and 361 bp 3$\sp\prime$ terminator of the gene. The ppConGlb1 cassette was then cloned into the Bin19 vector and transformed into tobacco plants. The results of immunoassays of the tobacco R$\sb1$ seeds using whole maize globulin antisera indicate that the GLB1 protein is expressed in immature tobacco seeds and GLB1 is correctly processed to its mature form.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Crop Sciences

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2011

Author dc:creator

• Liu, Siqing

Contributors dc:contributor

• Briskin, Donald P.

• Agriculture, Agronomy
• Biology, Genetics

Statement dc:rights

• Copyright 1994 Liu, Siqing

Language dc:language

eng

Identifier

AAI9503258
(UMI)AAI9503258

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/21028