Determination of hydrogen peroxide using immobilized chemiluminescence reagents and flow injection analysis

In this work, use of immobilized luminol for the CL determination of hydrogen peroxide using flow injection analysis (FIA) was investigated. Nylon was initially chosen as the immobilization support because of its chemical inertness. Luminol was covalently immobilized on the inside of nylon tubing and used for FIA determination of peroxide using solution-phase hemin as catalyst (detection limit = 0.4 μM, relative standard deviation (rsd) = 2.5%). Next, horseradish peroxidase (HRP) was immobilized on nylon membranes. Luminol was immobilized on to an anion exchange resin and packed in a column. When used in the FIA mode, stoichiometric amounts of luminol could be released from the column by injecting another anion with higher affinity towards the resin. The pH downstream of the column could also be controlled by the amount of anion injected upstream of the column. Such columns were used in a single channel FIA scheme for the determination of peroxide in conjunction with immobilized HRP as catalyst (detection limit = 8 μM, mean rsd = 0.8%). The experiment was extended for the determination of glucose by using co-immobilized HRP and glucose oxidase (detection limit = 25 μM). The problem presented by real samples was solved using a sample pre-treatment technique.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Chemistry

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2011

Author dc:creator

• Rao, Narasimha Murthy

Contributors dc:contributor

• Nieman, Timothy A.

• Chemistry, Analytical

Statement dc:rights

• Copyright 1990 Rao, Narasimha Murthy

Language dc:language

eng

Identifier

AAI9114378
(UMI)AAI9114378

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/20998