University of Illinois at Urbana-Champaign
Transcription termination by bacteriophage T7 RNA polymerase at rho-independent terminators
Abstract
dc:descriptionTo investigate the mechanism of transcription termination by bacteriophage RNA polymerases, templates encoding variants of the transcription-termination structure in the regulatory region of the threonine (thr) operon of Escherichia coli were used. The thr terminator comprises two distinct structural elements: a G+C-rich inverted repeat, which encodes an RNA hairpin structure, and A+T-rich regions, one of which contains a continuous sequence of template deoxyadenosine residues within which the transcript terminates. Fourteen terminator variants were analyzed by T7 RNA polymerase and we find that not only the hairpin structure itself but also its sequence influences termination. Furthermore, the formation of a hairpin in the RNA encoded by the A+T-rich regions of the attenuator is not mandatory for termination.
Degree
thesis:*- Name thesis:degree_name
- Ph.D.
- Level thesis:degree_level
- Dissertation
- Discipline thesis:degree_discipline
- Biochemistry
- Grantor
- University of Illinois at Urbana-Champaign
- Year dc:date
- 2011
Author and committee
dc:creator, dc:contributor.*- Author dc:creator
-
- Jeng, Shih-Tong
- Contributors dc:contributor
-
- Gumport, Richard I.
Subjects
dc:subject × 1Rights
dc:rights- Statement dc:rights
-
- Copyright 1991 Jeng, Shih-Tong
- Language dc:language
- eng
Identifiers
dc:identifier.*- Identifier
-
AAI9136627
(UMI)AAI9136627 - OAI identifier oai:identifier
- oai:www.ideals.illinois.edu:2142/20724