Applications of mass spectrometry to protein characterization

The application of mass spectrometry as a protein sequencing method is demonstrated by the study of bovine kidney aldose reductase. The protein is blocked at the N-terminus and is not amenable to Edman degradation. Using a strategy of homology comparison, the amino acid sequence was determined solely by mass spectrometric methods. The nature of aldose reductase oxidation was also identified.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Chemistry

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2011

Author dc:creator

• Huang, Rong

Contributors dc:contributor

• Rinehart, Kenneth L., Jr.

• Chemistry, Analytical
• Chemistry, Biochemistry

Statement dc:rights

• Copyright 1995 Huang, Rong

Language dc:language

eng

Identifier

AAI9624370
(UMI)AAI9624370

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/20686