Efficient production of doubled haploid plants through antimicrotubule agent treatment of anther-derived maize callus and the RFLP analysis of the maize anther culture response

Antimicrotubule agents, colchicine, amiprophos-methyl (APM), pronamide, oryzalin and trifluralin were used to induce chromosome doubling of maize anther-derived haploid callus before plant regeneration. Without treatment with these agents, all the plants regenerated from the callus were haploid. More than 50% of the plants regenerated from the callus treated with 0.625 or 1.25 uM colchicine for 1 to 3 days, 5 to 10 uM APM or 10 uM pronamide for 3 days were doubled haploids. These treatments had no effect in callus growth and plant regeneration. Oryzalin and trifluralin either severely inhibited the growth of regenerable callus or did not induce chromosome doubling effectively at lower concentrations. The results from field evaluation of the progeny of the doubled haploid plants from colchicine, APM and pronamide treatments suggest that no more than usual somaclonal variation was induced by these agents. Thus maize anther-derived haploid callus should be treated with these agents before plant regeneration in order to obtain a high frequency of doubled haploid plants.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Crop Sciences

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2011

Author dc:creator

• Wan, Yuechun

Contributors dc:contributor

• Widholm, Jack M.

• Agriculture, Agronomy
• Biology, Molecular
• Biology, Genetics

Statement dc:rights

• Copyright 1992 Wan, Yuechun

Language dc:language

eng

Identifier

AAI9215903
(UMI)AAI9215903

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/20483