Cytokinins tolerance and in vitro selection for dwarfism in apple (Malus x domestica Borkh.)

A reliable in vitro shoot regeneration system was developed for leaf segments of six apple (Malus Xdomestica Borkh) cultivars ('Wijcik', 'McIntosh', 'Macspur', 'Regal Gala' and 'M 26') grown in vitro using modified Murashige and Skoog (MS) medium supplemented with N-phenyl-N$\sp\prime$ 1,2,3,-Thidiazuron (TDZ). TDZ was found superior to benzylaminopurine (BAP) in promoting adventitious shoot formation. The optimum levels of TDZ ranged from 2 to 4 μM whereas, the optimum levels of BAP occurred between 4 to 12 μM depending on cultivar. Naphthaleneaceticid (NAA) was not necessary for adventitious shoot formation. In general, 3 to 4 weeks of dark incubation increased regenerate capacity of apple cultivars. The optimum levels of sucrose for regeneration were between 20 to 40 grams. Overall, no significant interaction was found between TDZ and dark period and between TDZ and sucrose to affect shoot regeneration. In most of the cultivars large leaves regenerated better than small ones. More adventitious shoots were obtained by plating the leaf segments on the medium with their abaxial surface uppermost. Regenerative capacity decreased as the number of times the a single shoot had been subcultured increased. Most of the adventitious shoots originated from cut edge of leaf segments, therefore the leaf mid segment was the most productive.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Crop Sciences

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2011

Author dc:creator

• Sarwar, Mushtaq

Contributors dc:contributor

• Skirvin, Robert M.

• Agriculture, Plant Culture
• Biology, Plant Physiology

Statement dc:rights

• Copyright 1996 Sarwar, Mushtaq

Language dc:language

eng

Identifier

9780591088434
AAI9702657
(UMI)AAI9702657

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/20474