Clinical and pathophysiologic studies of increased alkaline phosphatase isoenzymes in the liver and serum of dogs

The clinical and pathophysiologic conditions necessary for liver alkaline phosphatase isoenzymes to increase in canine blood were examined. It was first shown by a clinical study that increased serum activity of the corticosteroid-induced isoenzyme of alkaline phosphatase (CALP), which is unique to dogs, occurs in many different disease states. Further studies were performed to determine the possible underlying mechanisms responsible for this increase. Using gas chromatography/mass spectrometry it was shown that both CALP and liver alkaline phosphatase (LALP) purified from canine serum, contain myo-inositol residues in near equimolar amounts to the respective alkaline phosphatase monomer. This is consistent with release of alkaline phosphatase from hepatocyte membranes by endogenous glycosyl-phosphatidylinositol phospholipase D (GPI-PLD). Release of CALP and LALP into serum by GPI-PLD activity was supported by Western blot analyses using anti-cross reacting determinant antibodies and non-denaturing polyacrylamide gel electrophoresis.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Pathobiology

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2011

Author dc:creator

• Solter, Philip Frederick

Contributors dc:contributor

• Hoffmann, Walter E.

• Biology, Veterinary Science

Statement dc:rights

• Copyright 1994 Solter, Philip Frederick

Language dc:language

eng

Identifier

AAI9512555
(UMI)AAI9512555

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/20188