Genetic and biochemical analysis of the colicin I receptor gene of Escherichia coli: Structural organization and transcriptional control mechanisms

The cir gene, which encodes the colicin I receptor protein, is one of several outer membrane proteins synthesized by Escherichia coli in response to iron limitation. The location, organization, and regulation of cir and its neighboring genes were examined by a molecular genetic approach. Following cloning of the cir region, physical mapping and complementation analysis established the position of cir between mgl and nfo on the E. coli chromosome. A gene encoding a 32,000-dalton protein was located downstream of and adjacent to cir, was not part of the same transcriptional unit, and was not regulated by iron. The Cir protein was processed by removal of a signal peptide and the mature form had an amino terminal sequence which closely resembled that of other TonB-dependent proteins.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Microbiology

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2011

Author dc:creator

• Griggs, David William

Contributors dc:contributor

• Konisky, Jordan

• Biology, Molecular
• Biology, Microbiology

Statement dc:rights

• Copyright 1989 Griggs, David William

Language dc:language

eng

Identifier

AAI8916254
(UMI)AAI8916254

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/20009