Effects of in vivo dexamethasone sodium phosphate on in vitro equine neutrophil function

Neutrophil function was evaluated in vitro 24 and 48 hours after administration of a single IV dose of dexamethasone sodium phosphate (0.088 mg/kg) to adult horses. Neutrophils were separated from whole blood using a two-step Percoll gradient. Purified neutrophils from dexamethasone-treated horses had impaired ability to kill Staphylococcus aureus as compared to pretreatment and normal horse neutrophils. Neither phagocytosis, iodination, or phagosome-lysosome fusion was affected by treatment. Hydrogen peroxide production, measured both spectrophotometrically and by flow cytometry, was not affected by treatment. Lysosomal enzyme activity for alkaline phosphatase, acid phosphatase, lysozyme, β-glucuronidase, and myeloperoxidase was quantitated and was not affected by treatment.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Pathobiology

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2011

Author dc:creator

• Sturtevant, Faye Coleman

• Agriculture, Animal Pathology

Statement dc:rights

• Copyright 1989 Sturtevant, Faye Coleman

Language dc:language

eng

Identifier

AAI9011040
(UMI)AAI9011040

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/19795