University of Illinois at Urbana-Champaign
Stereochemistry, isotope effects, and inhibition of terpene biosynthesis: I. Stereospecificity and mechanism of monoterpene cyclases. II. Inhibition by heteroatom analogs of carbocation intermediates
Abstract
dc:descriptionStereospecificity and mechanism of monoterpene cyclases have been investigated by use of deuterium and tritium labeled substrates. The stereochemistry of the terminating deprotonation in limonene biosynthesis was elucidated by radiochemical degradation of ($\sp3$H) carvoximes. Limonene cyclases extracted from Citrus sinensis and Perilla frutescens effected regiospecific deprotonation, but pinene cyclases I and II from Salvia officinalis deprotonate non-regiospecifically. Deuterium isotope effects on the monoterpene product distribution from incubations of (1-$\sp3$H;8,9-$\sp2$H$\sb6$) GPP with the pinene cyclases are too small to explain by isotopically sensitive branching between limonene and the bicyclic monoterpenes.
Degree
thesis:*- Name thesis:degree_name
- Ph.D.
- Level thesis:degree_level
- Dissertation
- Discipline thesis:degree_discipline
- Chemistry
- Grantor
- University of Illinois at Urbana-Champaign
- Year dc:date
- 2011
Author and committee
dc:creator, dc:contributor.*- Author dc:creator
-
- Pyun, Hyung-Jung
- Contributors dc:contributor
-
- Coates, Robert M.
Subjects
dc:subject × 2Rights
dc:rights- Statement dc:rights
-
- Copyright 1992 Pyun, Hyung-Jung
- Language dc:language
- eng
Identifiers
dc:identifier.*- Identifier
-
AAI9215873
(UMI)AAI9215873 - OAI identifier oai:identifier
- oai:www.ideals.illinois.edu:2142/19639