Initiation of translation of the small RNA of the cowpea strain of tobacco mosaic virus

Several features of the initiation of translation of CcTMV-SRNA were investigated. First, the site of initiation of translation was found to be the first AUG in the 5$\sp\prime$-terminal sequence m$\sp7$GpppGUAUUUGAUGAUGGCA ... despite the fact that this AUG codon is not found within a favorable PuXXAUGG sequence. Second,analysis of the site of initiation of translation of mutant tCcTMV-SRNAs showed that the region of the tandem AUG's must contain some feature which directs the ribosome to initiate translation within that region and that the tandem AUGs themselves may be important for the actual selection of the correct translation initiator AUG codon. Third, investigation of the efficiency of the initiation of translation of the mutant tCcTMV-SRNAs showed that little if any relationship exists between the translational efficiency of the mutant tCcTMV-SRNAs and the agreement, of the sequences surrounding their translation initiation codons, with the PuXXAUGG consensus sequence. Finally, investigation of the translation of the mutant tCcTMV-SRNAs in the presence of $\sp{35}$SMet-tRNA$\sb{\rm i}\sp{\rm met}$ and $\sp{35}$S-Met-tRNA$\sb{\rm m}\sp{\rm met}$ produced unexpected results consistent with the miscoding of AAG codons to incorporate methionine under the conditions used.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Biochemistry

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2011

Author dc:creator

• Prouty, Rebecca E.

Contributors dc:contributor

• Clark, John M., Jr.

• Biology, Molecular
• Chemistry, Biochemistry

Statement dc:rights

• Copyright 1992 Prouty, Rebecca E.

Language dc:language

eng

Identifier

AAI9236572
(UMI)AAI9236572

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/19601