Genetic relatedness of the family Rhodospirillaceae: Genome plasticity, chromosome heterogeneity, and chalcogenic oxyanion reductase activity in Rhodobacter sphaeroides

"Intrinsic high-level resistance (HLR) to tellurite (TeO$\sb3\sp{2-}),$ selenite (SeO$\sb3\sp{2-}),$ and at least fifteen other chalcogenic oxides and oxyanions has been demonstrated in the facultative photoheterotrophic bacterium, Rhodobacter (Rba.) sphaeroides. Detailed analyses have shown that HLR to at least one class of these oxyanions, the ""tellurite class"" (e.g., \rm TeO\sb4\sp{2-}, TeO\sb3\sp{2-}, SeO\sb4\sp{2-}, SeO\sb3\sp{2-}, and $\rm Rh\sb2O\sb3),$ occurred via a metalloid oxyanion reductase (MORase), and resulted in elemental deposition in the cytoplasmic membrane, and concomitant H$\sb2$ evolution during photoheterotrophic growth in the presence of these oxyanions. Based on the analysis of TnphoA-derived mutants, the periplasmic-localized dimethyl sulfoxide/trimethylamine-N-oxide reductase (which has been isolated and purified to homogeneity) is not involved in the reduction of these oxyanions. Examination of other Rba. sphaeroides mutants, however, has determined the obligate requirement for an intact CO$\sb2$ fixation pathway and the presence of a functional photosynthetic electron transport chain to effect HLR to TeO$\sb3\sp{2-}$-class oxyanions under photosynthetic growth, and functional cytochromes b, c\sb1 and $c\sb2$ to facilitate HLR under aerobic growth conditions."

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Microbiology

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2011

Author dc:creator

• Moore, Mark David

Contributors dc:contributor

• Kaplan, Samuel
• Maloy, Stanley R.

• Biology, Molecular
• Biology, Genetics
• Biology, Microbiology

Statement dc:rights

• Copyright 1994 Moore, Mark David

Language dc:language

eng

Identifier

AAI9503276
(UMI)AAI9503276

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/18940