Multimodal imaging monitoring of key physiological changes of peripheral arterial disease

Peripheral Arterial Disease (PAD) limits blood flow to the lower extremities, causes functional impairment, and in extreme cases, can lead to amputation. While PAD is widely studied, there is still a large gap between preclinical research and clinical translation. This is due in part to the lack of research standardization in preclinical studies, an inadequate resemblance of the animals’ model to the actual disease in humans, and the complexity of the disease’s mechanisms. In this project, we utilized a type I diabetic mice model to establish a working platform that allows us and other researchers and scientists to standardize the preclinical animal studies of PAD. Within this platform, we will highlight critical physiological events that we term “landmarks.” To define these landmarks, we use a multimodal imaging approach starting with Laser Speckle Contrast Imaging (LSCI) and Power Doppler ultrasound for perfusion (superficial and deep tissue, respectively); followed by fluorescent imaging of hypoxia and imaging both angiogenesis and metabolism using molecular PET probes. Briefly, we found that building such a multimodal imaging approach allows us to understand vascular recovery in a holistic matter. However, most importantly, with this method, we will be able to determine the changes in the “landmarks” following the therapeutic intervention.

Name thesis:degree_name

M.S.

Level thesis:degree_level

Thesis

Discipline thesis:degree_discipline

Bioengineering

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2020

Author dc:creator

• Medina Almora, Denise

Contributors dc:contributor

• Dobrucki, Wawrzyniec L

• peripheral arterial disease, angiogenesis, perfusion, diabetes, imaging, multimodal imaging.

Statement dc:rights

• Copyright 2020 by Denise Medina Almora. All rights reserved.

Language dc:language

en

Handle dc:identifier

http://hdl.handle.net/2142/108724

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/108724