The Characterization of Osteocyte Apoptosis in a Mouse Model of Orthodontic Tooth Movement

<p>Background: Osteocyte apoptosis is regularly observed in the alveolar bone during orthodontic tooth movement (OTM). In this study, we wanted to further investigate its occurrence and localization.</p> <p>Method: Sixteen 10-12 week old male CD1 mice were loaded with NiTi springs between the right first maxillary molar and incisor delivering 10-12g of force. Left hemimaxillae served as unloaded controls. Four mice were sacrificed at each of the following time points: 6 hours, 12 hours, 3 days and 7 days after loading. Apoptosis was detected by TUNEL assay and caspase-3 and RipK-1 were detected by immunostaining on histological sections.</p> <p>Results: The ratio of TUNEL-positive osteocytes was increased on the compression side at 12 hours and, to a lesser extent, 3 days. There was a trend for increased caspase-3 expression at 12 hours, but RipK-1 expression levels did not follow this same trend.</p> <p>Conclusions: Caspase-3 expression correlates with increased osteocyte apoptosis during OTM. These results provide a better understanding of the molecular mechanism of osteocyte death during OTM.</p>

Name thesis:degree_name

Master of Dental Science

Discipline thesis:degree_discipline

Dental Science

Year dc:date.available

2012

Author dc:creator

• Moin, Sogole S

Contributors dc:contributor

• Ravindra Nanda; Ivo Kalajzic
• Flavio Uribe

• biology of orthodontic tooth movement
• osteocyte apoptosis
• animal study

Repository record dc:identifier

https://digitalcommons.lib.uconn.edu/gs_theses/298

OAI identifier oai:identifier

oai:digitalcommons.lib.uconn.edu:gs_theses-1331