Background: Osteocyte apoptosis is regularly observed in the alveolar bone during orthodontic tooth movement (OTM). In this study, we wanted to further investigate its occurrence and localization.
Method: Sixteen 10-12 week old male CD1 mice were loaded with NiTi springs between the right first maxillary molar and incisor delivering 10-12g of force. Left hemimaxillae served as unloaded controls. Four mice were sacrificed at each of the following time points: 6 hours, 12 hours, 3 days and 7 days after loading. Apoptosis was detected by TUNEL assay and caspase-3 and RipK-1 were detected by immunostaining on histological sections.
Results: The ratio of TUNEL-positive osteocytes was increased on the compression side at 12 hours and, to a lesser extent, 3 days. There was a trend for increased caspase-3 expression at 12 hours, but RipK-1 expression levels did not follow this same trend.
Conclusions: Caspase-3 expression correlates with increased osteocyte apoptosis during OTM. These results provide a better understanding of the molecular mechanism of osteocyte death during OTM.
","abstract_html":"<p>Background: Osteocyte apoptosis is regularly observed in the alveolar bone during orthodontic tooth movement (OTM). In this study, we wanted to further investigate its occurrence and localization.</p> <p>Method: Sixteen 10-12 week old male CD1 mice were loaded with NiTi springs between the right first maxillary molar and incisor delivering 10-12g of force. Left hemimaxillae served as unloaded controls. Four mice were sacrificed at each of the following time points: 6 hours, 12 hours, 3 days and 7 days after loading. Apoptosis was detected by TUNEL assay and caspase-3 and RipK-1 were detected by immunostaining on histological sections.</p> <p>Results: The ratio of TUNEL-positive osteocytes was increased on the compression side at 12 hours and, to a lesser extent, 3 days. There was a trend for increased caspase-3 expression at 12 hours, but RipK-1 expression levels did not follow this same trend.</p> <p>Conclusions: Caspase-3 expression correlates with increased osteocyte apoptosis during OTM. These results provide a better understanding of the molecular mechanism of osteocyte death during OTM.</p>","abstract_has_math":false,"creators":["Moin, Sogole S"],"institution":null,"degree_name":"Master of Dental Science","degree_level":null,"degree_discipline":"Dental Science","degree_department":null,"school":null,"contributors":["Ravindra Nanda; Ivo Kalajzic","Flavio Uribe"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2012,"date_issued":"2012-06-18T07:00:00Z","date_published":"2012-06-18T07:00:00Z","updated_at":"2026-07-24T06:31:50Z","subjects":["biology of orthodontic tooth movement","osteocyte apoptosis","animal study"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://digitalcommons.lib.uconn.edu/gs_theses/298","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Ravindra Nanda; Ivo Kalajzic","Flavio Uribe"]},{"key":"dc:creator","label":"Author","values":["Moin, Sogole S"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.available","label":"Dc Date Available","values":["2013-05-30T07:00:00Z"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Dental Science"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Dental Science"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["biology of orthodontic tooth movement","osteocyte apoptosis","animal study"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://digitalcommons.lib.uconn.edu/gs_theses/298"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Background: Osteocyte apoptosis is regularly observed in the alveolar bone during orthodontic tooth movement (OTM). In this study, we wanted to further investigate its occurrence and localization.
Method: Sixteen 10-12 week old male CD1 mice were loaded with NiTi springs between the right first maxillary molar and incisor delivering 10-12g of force. Left hemimaxillae served as unloaded controls. Four mice were sacrificed at each of the following time points: 6 hours, 12 hours, 3 days and 7 days after loading. Apoptosis was detected by TUNEL assay and caspase-3 and RipK-1 were detected by immunostaining on histological sections.
Results: The ratio of TUNEL-positive osteocytes was increased on the compression side at 12 hours and, to a lesser extent, 3 days. There was a trend for increased caspase-3 expression at 12 hours, but RipK-1 expression levels did not follow this same trend.
Conclusions: Caspase-3 expression correlates with increased osteocyte apoptosis during OTM. These results provide a better understanding of the molecular mechanism of osteocyte death during OTM.
"]},{"key":"dc:title","label":"Title","values":["The Characterization of Osteocyte Apoptosis in a Mouse Model of Orthodontic Tooth Movement"]}]}],"canonical_facts":{"dc:contributor":["Ravindra Nanda; Ivo Kalajzic","Flavio Uribe"],"dc:creator":["Moin, Sogole S"],"dc:date.available":["2013-05-30T07:00:00Z"],"dc:description.abstract":["Background: Osteocyte apoptosis is regularly observed in the alveolar bone during orthodontic tooth movement (OTM). In this study, we wanted to further investigate its occurrence and localization.
Method: Sixteen 10-12 week old male CD1 mice were loaded with NiTi springs between the right first maxillary molar and incisor delivering 10-12g of force. Left hemimaxillae served as unloaded controls. Four mice were sacrificed at each of the following time points: 6 hours, 12 hours, 3 days and 7 days after loading. Apoptosis was detected by TUNEL assay and caspase-3 and RipK-1 were detected by immunostaining on histological sections.
Results: The ratio of TUNEL-positive osteocytes was increased on the compression side at 12 hours and, to a lesser extent, 3 days. There was a trend for increased caspase-3 expression at 12 hours, but RipK-1 expression levels did not follow this same trend.
Conclusions: Caspase-3 expression correlates with increased osteocyte apoptosis during OTM. These results provide a better understanding of the molecular mechanism of osteocyte death during OTM.
"],"dc:identifier":["https://digitalcommons.lib.uconn.edu/gs_theses/298"],"dc:subject":["biology of orthodontic tooth movement","osteocyte apoptosis","animal study"],"dc:title":["The Characterization of Osteocyte Apoptosis in a Mouse Model of Orthodontic Tooth Movement"],"thesis:degree_discipline":["Dental Science"],"thesis:degree_name":["Master of Dental Science"]},"updated_at":"2026-07-24T06:31:50Z"}