Construction of an ABF-1 inducible expression cell line utilized to conduct a microarray analysis

<p>ABF-1 is a human class ll basic helix-loop-helix transcription factor that is expressed predominately in EBV immortalized and activated B lymphocytes. A human cell line was stably transfected with a tetracycline regulated ABF-1 expression vector. The cell line revealed tight regulation of ABF-1 expression following stable incorporation of the vector into the genomic DNA. Upon induction of ABF-1 expression, the cell line exhibited a dramatic growth rate decrease. In order to monitor genes regulated by ABF-1, cells were collected both before and after induced ABF-1 expression and subjected to a microarray analysis. Early interpretations of the microarray data support the findings that ABF-1 may be regulating gene expression in a manner that facilitates withdrawal from the cell cycle.</p>

Name thesis:degree_name

Master of Science (M.S.)

Level thesis:degree_level

Thesis - Pacific Access Restricted

Discipline thesis:degree_discipline

Biological Sciences

Year dc:date.available

2001

Author dc:creator

• O'Connell, Ryan M.

Contributors dc:contributor

• Craig Vierra

• Cell lines
• DNA microarrays
• Biology
• Life Sciences

Licence

• http://rightsstatements.org/vocab/InC/1.0/

Repository record dc:identifier

https://scholarlycommons.pacific.edu/uop_etds/556

OAI identifier oai:identifier

oai:scholarlycommons.pacific.edu:uop_etds-1555