Spectrophotometric and microbiological determination of the stability of sodium ampicillin as affected by pH

<p>The specific purpose of this investigation was to determine the effect of pH on the stability of ampicillin sodium for injection. Then, to correlate this information, a pH profile may be established to predict chemical stability with ampicillin sodium alone or when other drugs may be included in the admixture. With a pH profile the pharmacist could predict the length of time after which more than a 10 percent loss of activity would occur. Stability measurements were to be accomplished through a microbiological assay after adding ampicillin sodium to different buffer solutions. At the end of four hours, eight hours, and twelve hours, a quantitative determination was made to indicate to amount of ampicillin that is lost through chemical degradation.</p><p>The second purpose of this study was to test the validity of a spectrophotometric analysis of ampicillin, employing ultraviolet spectrophotometry to determine degradation of ampicillin at specific pH’s. All attempts were made to stimulate a clinical situation by adjusting the concentration, temperature, and illumination to the typical hospital environment.</p>

Name thesis:degree_name

Master of Science (M.S.)

Level thesis:degree_level

Thesis

Discipline thesis:degree_discipline

Pharmacy

Year dc:date.available

1972

Author dc:creator

• Raffanti, Enrico Frank

Contributors dc:contributor

• James C. King

• Antibiotics
• Hydrogen-ion concentration
• Chemistry
• Medicine and Health Sciences
• Physical Sciences and Mathematics

Licence

• http://rightsstatements.org/vocab/NKC/1.0/

Repository record dc:identifier

https://scholarlycommons.pacific.edu/uop_etds/417

OAI identifier oai:identifier

oai:scholarlycommons.pacific.edu:uop_etds-1416