The specific purpose of this investigation was to determine the effect of pH on the stability of ampicillin sodium for injection. Then, to correlate this information, a pH profile may be established to predict chemical stability with ampicillin sodium alone or when other drugs may be included in the admixture. With a pH profile the pharmacist could predict the length of time after which more than a 10 percent loss of activity would occur. Stability measurements were to be accomplished through a microbiological assay after adding ampicillin sodium to different buffer solutions. At the end of four hours, eight hours, and twelve hours, a quantitative determination was made to indicate to amount of ampicillin that is lost through chemical degradation.
The second purpose of this study was to test the validity of a spectrophotometric analysis of ampicillin, employing ultraviolet spectrophotometry to determine degradation of ampicillin at specific pH’s. All attempts were made to stimulate a clinical situation by adjusting the concentration, temperature, and illumination to the typical hospital environment.
","abstract_html":"<p>The specific purpose of this investigation was to determine the effect of pH on the stability of ampicillin sodium for injection. Then, to correlate this information, a pH profile may be established to predict chemical stability with ampicillin sodium alone or when other drugs may be included in the admixture. With a pH profile the pharmacist could predict the length of time after which more than a 10 percent loss of activity would occur. Stability measurements were to be accomplished through a microbiological assay after adding ampicillin sodium to different buffer solutions. At the end of four hours, eight hours, and twelve hours, a quantitative determination was made to indicate to amount of ampicillin that is lost through chemical degradation.</p><p>The second purpose of this study was to test the validity of a spectrophotometric analysis of ampicillin, employing ultraviolet spectrophotometry to determine degradation of ampicillin at specific pH’s. All attempts were made to stimulate a clinical situation by adjusting the concentration, temperature, and illumination to the typical hospital environment.</p>","abstract_has_math":false,"creators":["Raffanti, Enrico Frank"],"institution":null,"degree_name":"Master of Science (M.S.)","degree_level":"Thesis","degree_discipline":"Pharmacy","degree_department":null,"school":null,"contributors":["James C. King"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":1972,"date_issued":"1972-01-01T08:00:00Z","date_published":"1972-01-01T08:00:00Z","updated_at":"2026-07-24T05:36:21Z","subjects":["Antibiotics","Hydrogen-ion concentration","Chemistry","Medicine and Health Sciences","Physical Sciences and Mathematics"],"languages":[],"rights":[],"rights_urls":["http://rightsstatements.org/vocab/NKC/1.0/"],"identifier_entries":[]},"links":{"outbound_url":"https://scholarlycommons.pacific.edu/uop_etds/417","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["James C. King"]},{"key":"dc:creator","label":"Author","values":["Raffanti, Enrico Frank"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.available","label":"Dc Date Available","values":["1972-01-01T08:00:00Z"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Pharmacy"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science (M.S.)"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Antibiotics","Hydrogen-ion concentration","Chemistry","Medicine and Health Sciences","Physical Sciences and Mathematics"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:rights","label":"Dc Rights","values":["http://rightsstatements.org/vocab/NKC/1.0/"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://scholarlycommons.pacific.edu/uop_etds/417"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["The specific purpose of this investigation was to determine the effect of pH on the stability of ampicillin sodium for injection. Then, to correlate this information, a pH profile may be established to predict chemical stability with ampicillin sodium alone or when other drugs may be included in the admixture. With a pH profile the pharmacist could predict the length of time after which more than a 10 percent loss of activity would occur. Stability measurements were to be accomplished through a microbiological assay after adding ampicillin sodium to different buffer solutions. At the end of four hours, eight hours, and twelve hours, a quantitative determination was made to indicate to amount of ampicillin that is lost through chemical degradation.
The second purpose of this study was to test the validity of a spectrophotometric analysis of ampicillin, employing ultraviolet spectrophotometry to determine degradation of ampicillin at specific pH’s. All attempts were made to stimulate a clinical situation by adjusting the concentration, temperature, and illumination to the typical hospital environment.
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The second purpose of this study was to test the validity of a spectrophotometric analysis of ampicillin, employing ultraviolet spectrophotometry to determine degradation of ampicillin at specific pH’s. All attempts were made to stimulate a clinical situation by adjusting the concentration, temperature, and illumination to the typical hospital environment.
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