Bioaffinity-based Concept for Glyphosate Detection

In recent decades, there has been a growing emphasis on improving investigation capacities in the field of forensic toxicology. This study looks into the interaction between acetylcholinesterase (AChE) and glyphosate, specifically its potential suppression. The study optimizes parameters such as pH levels, enzyme concentrations, and substrate concentrations using the Ellman enzymatic assay. AChE activity is best maintained at 50 mU with 3 mM DTNB and 5 mM ACth, and storage at 4°C assures stability. The study also exposes glyphosate's inhibitory effect on AChE, demonstrating its potential hazards to human health and the environment. Comparisons with butyrylcholinesterase (BChE) demonstrate each enzyme's distinct stability and selectivity. These findings shed light on the complex interactions between glyphosate and cholinesterase, paving the path for breakthroughs in toxicology and related sciences.

Name thesis:degree_name

Master of Science

Level thesis:degree_level

Masters

Discipline thesis:degree_discipline

Forensic Science

Grantor

Texas Tech University

Year dc:date.issued

2023

Author dc:creator

• Haddock, Elizabeth (Lissa)

Chair dc:contributor.committeechair

• Halamek, Jan

Committee members dc:contributor.committeemember

• Ramkumar, S. S.
• Swed, Ori

• acetylcholinesterase
• organophosphate
• glyphosate
• enzymatic optimization
• enzyme stability
• glyphosate detection

Language dc:language.iso

en

Handle dc:identifier.uri

https://hdl.handle.net/2346/97430

OAI identifier oai:identifier

oai:ttu-ir.tdl.org:2346/97430