Imaging Chloride Homeostasis in Neurons

Intracellular chloride and pH are fundamental regulators of neuronal excitability and they are often co-modulated during excitation-inhibition activity. The study of their homeostasis requires simultaneous measurements in vivo in multiple neurons. Combining random mutagenesis screening, protein engineering and two-photon-imaging this thesis work led to the discovery of new chloride-sensitive GFP mutants and to the establishment of ratiometric imaging procedures for the quantitative combined imaging of intraneuronal pH and chloride. These achievements have been demonstrated in vivo in the mouse cortex, in real-time monitoring the dynamic changes of ions concentrations during epileptic-like discharges, and in glioblastoma primary cells, measuring osmotic swelling responses to various drugs treatment.

Grantor dc:publisher

Università degli studi di Trento

Year dc:date

2017

Author dc:creator

• Arosio, Daniele

Contributors dc:contributor

• Dalla Serra, Mauro

• Settore BIO/11 - Biologia Molecolare
• Settore FIS/07 - Fisica Applicata(Beni Culturali, Ambientali, Biol.e Medicin)
• Settore BIO/10 - Biochimica
• Settore BIO/13 - Biologia Applicata

Statement dc:rights

• info:eu-repo/semantics/openAccess
• license:Tutti i diritti riservati (All rights reserved)
• license uri:iris.PRI01

Language dc:language

eng

Identifier

http://dx.doi.org/10.15168/11572_368512
10.15168/11572_368512

OAI identifier oai:identifier

oai:iris.unitn.it:11572/368512