{"id":{"repo_id":"trento","oai_identifier":"oai:iris.unitn.it:11572/368512"},"canonical_url":"https://search.dev.ndltd.org/etd/trento/oai:iris.unitn.it:11572/368512","repository":{"repo_id":"trento","name":"Università degli Studi di Trento","base_url":"https://iris.unitn.it/oai/request"},"display":{"title":"Imaging Chloride Homeostasis in Neurons","abstract":"Intracellular chloride and pH are fundamental regulators of neuronal excitability and they are often co-modulated during excitation-inhibition activity. The study of their homeostasis requires simultaneous measurements in vivo in multiple neurons. Combining random mutagenesis screening, protein engineering and two-photon-imaging this thesis work led to the discovery of new chloride-sensitive GFP mutants and to the establishment of ratiometric imaging procedures for the quantitative combined imaging of intraneuronal pH and chloride. 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These achievements have been demonstrated in vivo in the mouse cortex, in real-time monitoring the dynamic changes of ions concentrations during epileptic-like discharges, and in glioblastoma primary cells, measuring osmotic swelling responses to various drugs treatment."]},{"key":"dc:title","label":"Title","values":["Imaging Chloride Homeostasis in Neurons"]}]}],"canonical_facts":{"dc:contributor":["Arosio, Daniele","Dalla Serra, Mauro"],"dc:creator":["Arosio, Daniele"],"dc:date":["2017"],"dc:description":["Intracellular chloride and pH are fundamental regulators of neuronal excitability and they are often co-modulated during excitation-inhibition activity. The study of their homeostasis requires simultaneous measurements in vivo in multiple neurons. 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These achievements have been demonstrated in vivo in the mouse cortex, in real-time monitoring the dynamic changes of ions concentrations during epileptic-like discharges, and in glioblastoma primary cells, measuring osmotic swelling responses to various drugs treatment."],"dc:identifier":["https://hdl.handle.net/11572/368512","http://dx.doi.org/10.15168/11572_368512","10.15168/11572_368512"],"dc:language":["eng"],"dc:publisher":["Università degli studi di Trento","place:TRENTO"],"dc:relation":["firstpage:1","lastpage:190","numberofpages:190"],"dc:rights":["info:eu-repo/semantics/openAccess","license:Tutti i diritti riservati (All rights reserved)","license uri:iris.PRI01"],"dc:subject":["Settore BIO/11 - Biologia Molecolare","Settore FIS/07 - Fisica Applicata(Beni Culturali, Ambientali, Biol.e Medicin)","Settore BIO/10 - Biochimica","Settore BIO/13 - Biologia Applicata"],"dc:title":["Imaging Chloride Homeostasis in Neurons"],"dc:type":["info:eu-repo/semantics/doctoralThesis"]},"updated_at":"2026-07-24T05:04:31Z"}