Mechanisms of Improved Renal Function Following Normothermic Ex-vivo Kidney Perfusion Preservation of Grafts Severely Damaged by Prolonged Warm Ischemia

Normothermic ex-vivo kidney perfusion (NEVKP) is an emerging technique for renal graft preservation. We investigated whether NEVKP could improve early function of severely injured donation-after-cardiovascular death (DCD) grafts to reduce the incidence of delayed graft function (DGF). First, we develop a large-animal model of significant renal dysfunction (SRD) that resembles clinical DGF following static cold storage (SCS). After comparing the function of these grafts following NEVKP-storage, we investigated potential mechanisms that may account for the observed outcomes by examining their gene expression pattern. To establish the SRD model, kidneys from 30kg-Yorkshire pigs were procured following increasing warm ischemic (WI) times of 0-120min (n=3-6/group) to mimic DCD. Following 8h of SCS and autotransplantation, animals were followed for 7-days. SRD was defined as oliguria

Department dc:contributor.department

Medical Science

Year dc:date.issued

2020

Author dc:creator

• Urbanellis, Peter

Advisors dc:contributor.advisor

• Selzner, Markus
• Robinson, Lisa A

• Donation-after-cardiovascular death
• Genomics
• Ischemia Reperfusion Injury
• Kidney Transplantation
• Organ Procurement
• Porcine models

Handle dc:identifier.uri

http://hdl.handle.net/1807/103458

OAI identifier oai:identifier

oai:utoronto.scholaris.ca:1807/103458