{"id":{"repo_id":"toronto-retro","oai_identifier":"oai:utoronto.scholaris.ca:1807/103458"},"canonical_url":"https://search.dev.ndltd.org/etd/toronto-retro/oai:utoronto.scholaris.ca:1807/103458","repository":{"repo_id":"toronto-retro","name":"University of Toronto","base_url":"https://utoronto.scholaris.ca/server/oai/request"},"display":{"title":"Mechanisms of Improved Renal Function Following Normothermic Ex-vivo Kidney Perfusion Preservation of Grafts Severely Damaged by Prolonged Warm Ischemia","abstract":"Normothermic ex-vivo kidney perfusion (NEVKP) is an emerging technique for renal graft preservation. We investigated whether NEVKP could improve early function of severely injured donation-after-cardiovascular death (DCD) grafts to reduce the incidence of delayed graft function (DGF). First, we develop a large-animal model of significant renal dysfunction (SRD) that resembles clinical DGF following static cold storage (SCS). After comparing the function of these grafts following NEVKP-storage, we investigated potential mechanisms that may account for the observed outcomes by examining their gene expression pattern. To establish the SRD model, kidneys from 30kg-Yorkshire pigs were procured following increasing warm ischemic (WI) times of 0-120min (n=3-6/group) to mimic DCD. Following 8h of SCS and autotransplantation, animals were followed for 7-days. SRD was defined as oliguria","abstract_html":"Normothermic ex-vivo kidney perfusion (NEVKP) is an emerging technique for renal graft preservation. We investigated whether NEVKP could improve early function of severely injured donation-after-cardiovascular death (DCD) grafts to reduce the incidence of delayed graft function (DGF). First, we develop a large-animal model of significant renal dysfunction (SRD) that resembles clinical DGF following static cold storage (SCS). After comparing the function of these grafts following NEVKP-storage, we investigated potential mechanisms that may account for the observed outcomes by examining their gene expression pattern. To establish the SRD model, kidneys from 30kg-Yorkshire pigs were procured following increasing warm ischemic (WI) times of 0-120min (n=3-6/group) to mimic DCD. Following 8h of SCS and autotransplantation, animals were followed for 7-days. SRD was defined as oliguria","abstract_has_math":false,"creators":["Urbanellis, Peter"],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":"Medical Science","school":null,"contributors":[],"advisors":["Selzner, Markus","Robinson, Lisa A"],"committee_chairs":[],"committee_members":[],"year":2020,"date_issued":"2020-11","date_published":"2020-11","updated_at":"2026-07-27T21:28:13Z","subjects":["Donation-after-cardiovascular death","Genomics","Ischemia Reperfusion Injury","Kidney Transplantation","Organ Procurement","Porcine models"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/1807/103458","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Selzner, Markus","Robinson, Lisa A"]},{"key":"dc:contributor.department","label":"Department","values":["Medical Science"]},{"key":"dc:creator","label":"Author","values":["Urbanellis, Peter"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2020-11"]},{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2020-11-30T19:55:06Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2020-11-30T19:55:06Z"]},{"key":"dc:date.issued","label":"Date","values":["2020-11"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Donation-after-cardiovascular death","Genomics","Ischemia Reperfusion Injury","Kidney Transplantation","Organ Procurement","Porcine models"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/1807/103458"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Normothermic ex-vivo kidney perfusion (NEVKP) is an emerging technique for renal graft preservation. We investigated whether NEVKP could improve early function of severely injured donation-after-cardiovascular death (DCD) grafts to reduce the incidence of delayed graft function (DGF). First, we develop a large-animal model of significant renal dysfunction (SRD) that resembles clinical DGF following static cold storage (SCS). After comparing the function of these grafts following NEVKP-storage, we investigated potential mechanisms that may account for the observed outcomes by examining their gene expression pattern. To establish the SRD model, kidneys from 30kg-Yorkshire pigs were procured following increasing warm ischemic (WI) times of 0-120min (n=3-6/group) to mimic DCD. Following 8h of SCS and autotransplantation, animals were followed for 7-days. SRD was defined as oliguria"]},{"key":"dc:description.degree","label":"Dc Description Degree","values":["Ph.D."]},{"key":"dc:title","label":"Title","values":["Mechanisms of Improved Renal Function Following Normothermic Ex-vivo Kidney Perfusion Preservation of Grafts Severely Damaged by Prolonged Warm Ischemia"]}]}],"canonical_facts":{"dc:contributor.advisor":["Selzner, Markus","Robinson, Lisa A"],"dc:contributor.department":["Medical Science"],"dc:creator":["Urbanellis, Peter"],"dc:date":["2020-11"],"dc:date.accessioned":["2020-11-30T19:55:06Z"],"dc:date.available":["2020-11-30T19:55:06Z"],"dc:date.issued":["2020-11"],"dc:description.abstract":["Normothermic ex-vivo kidney perfusion (NEVKP) is an emerging technique for renal graft preservation. We investigated whether NEVKP could improve early function of severely injured donation-after-cardiovascular death (DCD) grafts to reduce the incidence of delayed graft function (DGF). First, we develop a large-animal model of significant renal dysfunction (SRD) that resembles clinical DGF following static cold storage (SCS). After comparing the function of these grafts following NEVKP-storage, we investigated potential mechanisms that may account for the observed outcomes by examining their gene expression pattern. To establish the SRD model, kidneys from 30kg-Yorkshire pigs were procured following increasing warm ischemic (WI) times of 0-120min (n=3-6/group) to mimic DCD. Following 8h of SCS and autotransplantation, animals were followed for 7-days. SRD was defined as oliguria"],"dc:description.degree":["Ph.D."],"dc:identifier.uri":["http://hdl.handle.net/1807/103458"],"dc:subject":["Donation-after-cardiovascular death","Genomics","Ischemia Reperfusion Injury","Kidney Transplantation","Organ Procurement","Porcine models"],"dc:title":["Mechanisms of Improved Renal Function Following Normothermic Ex-vivo Kidney Perfusion Preservation of Grafts Severely Damaged by Prolonged Warm Ischemia"],"dc:type":["Thesis"]},"updated_at":"2026-07-27T21:28:13Z"}