Localization of Cytoskeletal Elements in Teleost Retinal Pigment Epithelium

The G.3.5 antigen (Mr = 100,000) has been localized in brain, cerebellum, skeletal, cardiac, and smooth muscle tissues using the G.3.5 mAb, and has been described as an IFAP on the basis of its co-localization and co-isolation with desmin. Through biochemical analysis, the G.3.5 antigen was found to contain a peptide sequence that is 80% homologous to the actin binding region of human sarcomeric a-actinin. Recent Studies have suggested that the G.3.5 antigen may be a novel isoform of a-actinin, and may function to cross-link [Fs to MFs. In this study, I have used epifluorescence labeling techniques on cryosectioned bluegill RPE that have either been PFA fixed or hexane prepared. Using confocal microscopy, the G.3.5 antigen appears to co-localize with IFs, but not the MFs of CMBs in frozen RPE sections. These results do not support a role for the G.3.5 antigen in cross-linking CMB MEFs to IFs in bluegill RPE. In similar preparations, labeling patterns of cytoplasmic α-actinin and the G.3.5 antigen were observed to be different, thus suggesting that the G.3.5 antigen is distinct from cytoplasmic α-actinin.

Name thesis:degree_name

Master of Science

Level thesis:degree_level

Masters

Discipline thesis:degree_discipline

Biology

Grantor

Southwest Texas State University

Year dc:date.issued

1997

Author dc:creator

• Zamora, David O.

Advisor dc:contributor.advisor

• Gardia, Dana M.

Committee members dc:contributor.committeemember

• Koke, Joseph R.
• McLean, Robert J. C.

• cytoskeletal proteins
• epithelial cells
• Osteichthyes

Language dc:language.iso

en

Handle dc:identifier.uri

https://hdl.handle.net/10877/23576

OAI identifier oai:identifier

oai:digital.library.txst.edu:10877/23576