Morphology of Capillary Endothelia in Dog Heart Visualized by Scanning Electron Microscopy: The Effects of Ischemia and AMP

Endocytotic transport may provide a mechanism for myocyte uptake of agents which ameliorate the affects of myocardial ischemia. Examination of the inner capillary surface by transmission electron microscopy is tedious and slow, while use of scanning electron microscopy allows examining greater areas in less time. We explored methods to obtain oblique exposures of capillaries and provide sufficient resolution to observe endocytotic pits. We were partially successful using glutaraldehyde fixed tissue, treated with 302 glycerol, which was frozen-fractured under hexane at -65° C. The tissue was then dehydrated with acetone at ~25° C, critical-point dried and lightly coated with gold. Ultrastructural detail comparable with TEM images of heart tissue was obtained, but resolution appeared limited by the coating process. Because of this limitation, we were unable to verify stimulation of endocytosis by AMP in coronary capillary endothelia with scanning electron microscopy.

Name thesis:degree_name

Master of Science

Level thesis:degree_level

Masters

Discipline thesis:degree_discipline

Biology

Grantor

Southwest Texas State University

Year dc:date.issued

1986

Author dc:creator

• Kyle, Carol Tindall

Advisor dc:contributor.advisor

• Koke, Joseph R.

Committee members dc:contributor.committeemember

• Tomasso, Joseph R.
• Willms, Charles R.

• coronary heart disease
• endocytosis
• adenylic acid
• dogs

Language dc:language.iso

en

Handle dc:identifier.uri

https://hdl.handle.net/10877/20077

OAI identifier oai:identifier

oai:digital.library.txst.edu:10877/20077