{"id":{"repo_id":"texas-state","oai_identifier":"oai:digital.library.txst.edu:10877/20077"},"canonical_url":"https://search.dev.ndltd.org/etd/texas-state/oai:digital.library.txst.edu:10877/20077","repository":{"repo_id":"texas-state","name":"Texas State University","base_url":"https://digital.library.txst.edu/server/oai/request"},"display":{"title":"Morphology of Capillary Endothelia in Dog Heart Visualized by Scanning Electron Microscopy: The Effects of Ischemia and AMP","abstract":"Endocytotic transport may provide a mechanism for myocyte uptake of agents which ameliorate the affects of myocardial ischemia. Examination of the inner capillary surface by transmission electron microscopy is tedious and slow, while use of scanning electron microscopy allows examining greater areas in less time. We explored methods to obtain oblique exposures of capillaries and provide sufficient resolution to observe endocytotic pits. We were partially successful using glutaraldehyde fixed tissue, treated with 302 glycerol, which was frozen-fractured under hexane at -65° C. The tissue was then dehydrated with acetone at ~25° C, critical-point dried and lightly coated with gold. Ultrastructural detail comparable with TEM images of heart tissue was obtained, but resolution appeared limited by the coating process. Because of this limitation, we were unable to verify stimulation of endocytosis by AMP in coronary capillary endothelia with scanning electron microscopy.","abstract_html":"Endocytotic transport may provide a mechanism for myocyte uptake of agents which ameliorate the affects of myocardial ischemia. Examination of the inner capillary surface by transmission electron microscopy is tedious and slow, while use of scanning electron microscopy allows examining greater areas in less time. We explored methods to obtain oblique exposures of capillaries and provide sufficient resolution to observe endocytotic pits. We were partially successful using glutaraldehyde fixed tissue, treated with 302 glycerol, which was frozen-fractured under hexane at -65° C. The tissue was then dehydrated with acetone at ~25° C, critical-point dried and lightly coated with gold. Ultrastructural detail comparable with TEM images of heart tissue was obtained, but resolution appeared limited by the coating process. Because of this limitation, we were unable to verify stimulation of endocytosis by AMP in coronary capillary endothelia with scanning electron microscopy.","abstract_has_math":false,"creators":["Kyle, Carol Tindall"],"institution":"Southwest Texas State University","degree_name":"Master of Science","degree_level":"Masters","degree_discipline":"Biology","degree_department":null,"school":null,"contributors":[],"advisors":["Koke, Joseph R."],"committee_chairs":[],"committee_members":["Tomasso, Joseph R.","Willms, Charles R."],"year":1986,"date_issued":"1986-05","date_published":"1986-05","updated_at":"2026-07-27T21:22:32Z","subjects":["coronary heart disease","endocytosis","adenylic acid","dogs"],"languages":["en"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/10877/20077","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Koke, Joseph R."]},{"key":"dc:contributor.committeemember","label":"Committee Member","values":["Tomasso, Joseph R.","Willms, Charles R."]},{"key":"dc:creator","label":"Author","values":["Kyle, Carol Tindall"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2024-12-18T22:47:20Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2024-12-18T22:47:20Z"]},{"key":"dc:date.issued","label":"Date","values":["1986-05"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Masters"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science"]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["Southwest Texas State University"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["coronary heart disease","endocytosis","adenylic acid","dogs"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["en"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://hdl.handle.net/10877/20077"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Endocytotic transport may provide a mechanism for myocyte uptake of agents which ameliorate the affects of myocardial ischemia. Examination of the inner capillary surface by transmission electron microscopy is tedious and slow, while use of scanning electron microscopy allows examining greater areas in less time. We explored methods to obtain oblique exposures of capillaries and provide sufficient resolution to observe endocytotic pits. We were partially successful using glutaraldehyde fixed tissue, treated with 302 glycerol, which was frozen-fractured under hexane at -65° C. The tissue was then dehydrated with acetone at ~25° C, critical-point dried and lightly coated with gold. Ultrastructural detail comparable with TEM images of heart tissue was obtained, but resolution appeared limited by the coating process. Because of this limitation, we were unable to verify stimulation of endocytosis by AMP in coronary capillary endothelia with scanning electron microscopy."]},{"key":"dc:format","label":"Dc Format","values":["Text"]},{"key":"dc:format.medium","label":"Dc Format Medium","values":["1 file (.pdf)"]},{"key":"dc:title","label":"Title","values":["Morphology of Capillary Endothelia in Dog Heart Visualized by Scanning Electron Microscopy: The Effects of Ischemia and AMP"]}]}],"canonical_facts":{"dc:contributor.advisor":["Koke, Joseph R."],"dc:contributor.committeemember":["Tomasso, Joseph R.","Willms, Charles R."],"dc:creator":["Kyle, Carol Tindall"],"dc:date.accessioned":["2024-12-18T22:47:20Z"],"dc:date.available":["2024-12-18T22:47:20Z"],"dc:date.issued":["1986-05"],"dc:description.abstract":["Endocytotic transport may provide a mechanism for myocyte uptake of agents which ameliorate the affects of myocardial ischemia. Examination of the inner capillary surface by transmission electron microscopy is tedious and slow, while use of scanning electron microscopy allows examining greater areas in less time. We explored methods to obtain oblique exposures of capillaries and provide sufficient resolution to observe endocytotic pits. We were partially successful using glutaraldehyde fixed tissue, treated with 302 glycerol, which was frozen-fractured under hexane at -65° C. The tissue was then dehydrated with acetone at ~25° C, critical-point dried and lightly coated with gold. Ultrastructural detail comparable with TEM images of heart tissue was obtained, but resolution appeared limited by the coating process. Because of this limitation, we were unable to verify stimulation of endocytosis by AMP in coronary capillary endothelia with scanning electron microscopy."],"dc:format":["Text"],"dc:format.medium":["1 file (.pdf)"],"dc:identifier.uri":["https://hdl.handle.net/10877/20077"],"dc:language.iso":["en"],"dc:subject":["coronary heart disease","endocytosis","adenylic acid","dogs"],"dc:title":["Morphology of Capillary Endothelia in Dog Heart Visualized by Scanning Electron Microscopy: The Effects of Ischemia and AMP"],"dc:type":["Thesis"],"thesis:degree_discipline":["Biology"],"thesis:degree_level":["Masters"],"thesis:degree_name":["Master of Science"],"thesis:institution_name":["Southwest Texas State University"]},"updated_at":"2026-07-27T21:22:32Z"}